期刊
ANALYTICAL CHEMISTRY
卷 81, 期 9, 页码 3329-3333出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac900395x
关键词
-
资金
- National Natural Science Foundation [20573041, 20773044, 20873050]
- Program for Changjiang Scholars and Innovative Research Team in University [IRT0422]
- Program for New Century Excellent Talents in University [1306009]
- Development Program of the Science and Technology of Jilin Province [20060902-02]
- KAK-ENHI [470, 20043032]
- Ministry of Education, Culture, Sports, Science and Technology of Japan
- China Scholarship Council
We detected concentration-dependent surface-enhanced Raman scattering (SERS) spectra of several label-free proteins (lysozyme, ribonuclease B, avidin, catalase, and hemoglobin) for the first time in aqueous solutions. Acidified sulfate was used as an aggregation agent to induce high electromagnetic enhancement in SERS. Strong SERS spectra of simple and conjugated protein samples could easily be accessed after the pretreatment with the aggregation agent. The detection limits of the proposed method for lysozyme and catalase were as low as 5 mu g/ mL and 50 ng/mL, respectively. This detection protocol for label-free proteins has combined simplicity, sensitivity, and reproducibility and allows routine qualitative and relatively quantitative detections. Thus, it has great potential in practical high-throughput protein detections.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据