期刊
ANALYTICAL CHEMISTRY
卷 81, 期 5, 页码 2027-2031出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac9001172
关键词
-
资金
- Coulter Foundation
- NSF [CBET-0747105]
- Directorate For Engineering
- Div Of Chem, Bioeng, Env, & Transp Sys [0747105, 1016547] Funding Source: National Science Foundation
Droplet-based microfluidics has raised a lot of interest recently due to its wide applications to screening biological/chemical assays with high throughput. Despite the advances on droplet-based assays involving cells, gene delivery methods that are compatible with the droplet platform have been lacking. In this report, we demonstrate a simple microfluidic device that encapsulates cells into aqueous droplets and then electroporates the encapsulated cells. The electroporation occurs when the cell-containing droplets (in oil) flow through a pair of micro-electrodes with a constant voltage established in between. We investigate the parameters and characteristics of the electroporation. We demonstrate delivering enhanced green fluorescent protein (EGFP) plasmid into Chinese hamster ovary (CHO) cells. We envision the application of this technique to high-throughput functional genomics studies based on droplet microfluidics.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据