期刊
CLINICAL INFECTIOUS DISEASES
卷 45, 期 1, 页码 95-102出版社
OXFORD UNIV PRESS INC
DOI: 10.1086/518608
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During recent years, the usefulness of amoebal co-cultures as an alternative means of isolating and cultivating fastidious microorganisms has been increasingly recognized. While characterizing a collection of bacteria that had been isolated using this approach, we encountered an organism that, on preliminary analysis, appeared to be a gram-positive coccus. However, additional examination revealed that it was not a bacterium but rather, surprisingly, a virus. The dimensions of the virus particle ( diameter, 0.8 mm) and its genome size ( 1.2 Mb) are far more akin to those of bacteria than to those of previously recognized viruses. These characteristics, together with such features as the breadth and complexity of its gene content, challenge the current definition of a virus. Furthermore, the virus, now named Mimivirus, has been implicated as an agent of pneumonia in humans and, thus, should be considered a putative emerging pathogen.
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