期刊
ANALYTICAL CHEMISTRY
卷 81, 期 23, 页码 9633-9642出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac9016677
关键词
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资金
- U.S. Department of Homeland Security (DHS)
- Metro Washington Chapter of Achievement Rewards for College Scientists Foundation
- CPTI [R01 CA126189]
- NATIONAL CANCER INSTITUTE [R01CA126189] Funding Source: NIH RePORTER
MALDI mass spectrometry-based systems for rapid characterization of microorganisms in biodefense or medical diagnostics usually detect intact proteins in the 5000-20,000 Da range. To evaluate the reliability of species discrimination, and also for forensic applications, it is important that these biomarker proteins be identified. In the present study we apply high resolution tandem mass analysis on an Orbitrap and top-down bioinformatics to identify major biomarker proteins observed in MALDI spectra of intact bacteria for which little genomic or protein sequence information is available. The strategy depends on recognition of proteins with very high homology in related (sequenced) species, making it possible to place unsequenced organisms in their correct phylogenetic context. We show that this rapid proteomics based approach to phylogenetic characterization produces similar results to the traditional techniques, and may even be applied to target organisms of undetermined taxonomy. We further discuss important issues in combining genomics/proteomics databases and MALDI MS for the rapid characterization of microorganisms.
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