Effect of H/D Isotopomerization in the Assay of Resveratrol by Tandem Mass Spectrometry and Isotope Dilution Method

Resveratrol is a phytoalexin found in several plant tissues and present in wines, which is supplied as a nutritional supplement. Different studies have revealed its beneficial effects as anticancer, antiviral, neuroprotective, antiaging, and anti-inflammatory natural active principle. The assaying of resveratrol by mass spectrometry and isotope dilution method, using a stable [H-2(4)] analogue, has required a full elucidation of its gas-phase H/D isotopomerization. Either selected ion monitoring (SIM) or multiple reaction monitoring (MRM) methods have been used for the evaluation of the amount of resveratrol present in wine and plasma samples in the negative ionization mode. In all instances the acquired accuracy, limit of quantitation (LOQ), and limit of detection (LOD) are fit for the intended purpose of the assay.