期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 16, 期 11, 页码 27576-27588出版社
MDPI AG
DOI: 10.3390/ijms161126051
关键词
borneol; P-glycoprotein; brain microvascular endothelial cells; NF-kappa B; blood brain barrier
资金
- National Basic Research Program of China (973 Program) [2005CB523400]
P-glycoprotein (P-gp) on brain microvascular endothelial cells (BMECs) that form the blood brain barrier (BBB), influences transportation of substances between blood and brain. The objective of this study was to characterize the effects of borneol on P-gp efflux function on BBB and explore the potential mechanisms. We established an in vitro BBB model comprised of rat BMECs and astrocytes to measure the effects of borneol on the known P-gp substrates transport across BBB, and examined the function and expression of P-gp in BMECs and the signaling pathways regulating P-gp expression. Borneol increased intracellular accumulation of Rhodamine 123, enhanced verapamil and digoxin across the BBB in vitro model, and depressed mdr1a mRNA and P-gp expression. Borneol could activate nuclear factor-B (NF-B) and inhibition of NF-B with MG132 (carbobenzoxy-Leu-Leu-leucinal) and SN50 (an inhibitory peptide) obscuring the P-gp decreases induced by borneol. These data suggested that borneol depresses P-gp function in BMECs by a NF-B signaling medicated mechanism in a BBB in vitro model.
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