期刊
JOURNAL OF BACTERIOLOGY
卷 189, 期 13, 页码 4809-4814出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JB.01786-06
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The ybxF gene is a member of the streptomycin operon in a wide range of gram-positive bacteria. In Bacillus subtilis, it codes for a small basic protein (82 amino acids, pI 9.51) of unknown function. We demonstrate that, in B. subtilis, YbxF localizes to the ribosome, primarily to the 50S subunit, with dependence on growth phase. Based on three-dimensional structures of YbxF generated by homology modeling, we identified helix 2 as important for the interaction with the ribosome. Subsequent mutational analysis of helix 2 revealed Lys24 as crucial for the interaction. Neither the B. subtilis ybxF gene nor its paralogue, the ymxC gene, is essential, as shown by probing Delta ybxF, Delta ymxC, or Delta ybxF Delta ymxC double deletion strains in several functional assays.
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