期刊
ANALYTICAL CHEMISTRY
卷 80, 期 3, 页码 707-712出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac701910r
关键词
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资金
- Division Of Chemistry
- Direct For Mathematical & Physical Scien [840684] Funding Source: National Science Foundation
- NIBIB NIH HHS [R01 EB002189-08, R01 EB002189-07, R01 EB002189, R01 EB002189-06] Funding Source: Medline
- PHS HHS [R01 1056047] Funding Source: Medline
We here report or. the first example of an aptamer-based potentiometric sandwich assay of proteins. The measurements are based on CdS quantum dot labels of the secondary aptamer, which were determined with a novel solid-contact Cd2+-selective polymer membrane electrode after dissolution with hydrogen peroxide. The electrode exhibited cadmium ion detection limits of 100 pM in 100 mL samples and of 1 nM in 200 mu L microwells, using a calcium-selective electrode as a pseudoreference electrode. As a prototype example, thrombin was measured in 200 mu L samples with a lower detection limit of 0.14 nM corresponding: to 28 fmol of analyte. The results show great promise foil the potentiometric determination of proteins at very low concentrations in microliter samples.
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