4.8 Article

Exact analysis of ligand-induced dimerization of monomeric receptors

期刊

ANALYTICAL CHEMISTRY
卷 80, 期 14, 页码 5550-5555

出版社

AMER CHEMICAL SOC
DOI: 10.1021/ac800578w

关键词

-

向作者/读者索取更多资源

This paper analyzes the equilibria involved in the dimerization of monomeric receptors with homo-bifunctional ligands. We provide analytical expressions that can be used to estimate the concentration of each species present in a mixture of homo-bifunctional ligand and monomeric proteins, given initial conditions defining the total concentration of bivalent ligand [L-2](0), the total concentration of protein [P](0), one dissociation constant K-d, and a parameter to account for cooperativity alpha. We demonstrate that the fraction of protein present in a complex of two proteins and one bivalent ligand (P center dot L-2 center dot P) is maximized at [L-2](0) = K-d/2 + [P](0)/2.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.8
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据