期刊
ANALYTICAL CHEMISTRY
卷 80, 期 22, 页码 8382-8388出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac800857p
关键词
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资金
- National Natural Science Foundation of China [20875052]
- Natural Science Foundation of Shandong Province [Z20071331]
- Analytical Chemistry for Life Science
- Ministry of Education [KLACLS07001]
In the present study, an electrochemical sensing strategy for highly sensitive detection of small molecules was developed based on switching structures of aptamers from DNA/DNA duplex to DNA/target complex. A gold electrode was first modified with gold nanoparticles (AuNPs), and thiolated capture probe was immobilized onto the electrode via sulfur-gold affinity. Then, a sandwich-type strategy was employed, which involved a linker DNA containing antiadenosine aptamer sequence and reporter DNA loaded on AuNPs. In the presence of adenosine, the aptamer part bound with adenosine and folded to the complex structure. As a result, the reporter probes together with AuNPs were released into solution and reduced a decrease in peak current. With the enhancement effect of AuNPs, a detection limit as low as 1.8 x 10(-10) M for adenosine was achieved. The sensor exhibited excellent selectivity against other nucleosides and could be used to detect adenosine from real human serum samples.
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