期刊
ANALYTICAL CHEMISTRY
卷 80, 期 15, 页码 6018-6022出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac800726g
关键词
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资金
- Grants-in-Aid for Scientific Research [20310069] Funding Source: KAKEN
Real-time imaging of single-molecule fluorescence with a zero-mode waveguide (ZMW) was achieved. With modification of the ZMW geometry, the signal-to-background ratio is twice that obtainable with a conventional ZMW. The improved signal-to-background ratio makes it possible to visualize individual binding-release events between chaperonin GroEL and cochaperonin GroES at a concentration of 5 mu M. Two rate constants representing two-timer kinetics in the release of GroES from GroEL were measured with the ZMW, and the measurements agreed well with those made with a total internal reflection fluorescence microscopy. These results indicate that the novel ZMW makes feasible the direct observation of protein-protein interaction at an intracellular concentration in real time.
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