Solvent Signal as an NMR Concentration Reference

We propose that the NMR solvent signal be utilized as a universal concentration reference because most solvents can be observed by NMR and solvent concentrations can be readily calculated or determined independently. In particular, a highly protonated solvent such as water can serve as a primary concentration standard for its stability, availability, and ease of observation. The potential problems of radiation damping associated with a strong NMR signal can be alleviated by small pulse angle excitation. The solvent signal then can be detected by the NMR receiver with the same efficiency as a dilute analyte. We demonstrated that the analyte's proton concentration can be accurately determined from 4 mu M to more than 100 M, referenced by solvent (water) protons of concentrations more than 10 M. The proposed method is robust and indifferent to probe tuning and does not require any additional concentration standard.