期刊
ANALYTICAL CHEMISTRY
卷 80, 期 14, 页码 5367-5376出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac8003488
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资金
- NCRR NIH HHS [RR018522, P41 RR018522, P41 RR018522-06] Funding Source: Medline
- NIAID NIH HHS [Y1-AI-4894-01] Funding Source: Medline
Time-of-flight mass spectrometry (TOF MS) is increasingly used in proteomics research. Herein, we report on the development and characterization of a TOF MS instrument with improved sensitivity equipped with an electrodynamic ion funnel trap (IFT) that employs an automated gain control (AGC) capability. The IFr-TOF MS was coupled to a reversed-phase capillary liquid chromatography (RPLC) separation and evaluated in experiments with complex proteolytic digests. When applied to a global tryptic digest of Shewanella oneidensis proteins, an order of-magnitude increase in sensitivity compared to that of the conventional continuous mode of operation was achieved due to efficient ion accumulation prior to TOF MS analysis. As a result of this sensitivity improvement and related improvement in mass measurement accuracy, the number of unique peptides identified in the AGC-IFT mode was 5-fold greater than that obtained in the continuous mode.
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