期刊
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
卷 35, 期 3, 页码 693-701出版社
SPANDIDOS PUBL LTD
DOI: 10.3892/ijmm.2015.2062
关键词
high mobility group A1; transforming growth factor-beta 1; breast cancer; human epidermal growth factor receptor 2
资金
- National Natural Science Foundation of P.R. China [81272355, 31200573, 81170807, 81372824]
- Hunan Provincial Natural Science Foundation of China [12JJ3116]
- Education Department of Hunan Province Youth Fund [12B108]
Transforming growth factor-beta 1 (TGF-beta 1) signaling and high mobility group A (HMGA1) are known to play essential roles in the progression of breast cancer by inducing epithelial-mesenchymal transition. However, the correlation between TGF-beta 1 and HMGA1 in breast cancer cell is not yet well understood. In this study, we determined the effects of TGF-beta 1 on HMGA1 expression in breast cancer cells and examined the role of HMGA1 in breast cancer progression. Our results demonstrated that TGF-beta 1 induced the expression of HMGA1 in both MCF-7 and MDA-MB-231 breast cancer cells, as shown by RT-qPCR and immunofluorescence staining; however, the TGF-beta 1-induced expression of HMGA was blocked by treatment of the cells with phosphatidylinositol-3 kinase (PI3K) signaling inhibitors. Moreover, the HMGA1 promoter activity was found to be activated by TGF-beta 1 in the MCF-7 and MDA-MB-231 cells and we found that specificity protein 1 (Sp1) was involved in the TGF-beta 1-induced HMGA1 promoter activity, as shown by luciferase activity assay. Furthermore, the enforced expression of HMGA1 by transfection with a HMGA1 promoter enhanced cellular oncogenic properties, including proliferation, migration and invasion, and a tissue microarray revealed that breast tumors expressing human epidermal growth factor receptor 2 (HER2) showed higher expression levels of HMGA1 (P=0.007). In addition, higher HMGA1 expression levels were also observed in the ductal breast cancer cases compared with the lobular breast cancer cases (P=0.000). These findings establish the first link between HMGA1 and TGF-beta 1 in breast cancer, providing further evidence of the pivotal role of HMGA1 in breast cancer progression.
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