期刊
PLANT CELL REPORTS
卷 26, 期 7, 页码 889-894出版社
SPRINGER
DOI: 10.1007/s00299-006-0303-6
关键词
tobacco BY-2 cells; cell cycle; cytokinesis; Schizosaccharomyces pombe
The molecular mechanisms controlling cytokinesis in plant cell division cycle remains largely unknown. In this study, a functional approach was taken to identify genes that may play roles in cytokinesis in tobacco BY-2 cells, using fission yeast as the host organism. A total of 22 BY-2 genes that perturbed the terminal stage of cell division when ectopically expressed in yeast cells were isolated, among which, several encode for uncharacterized genes. Additionally, RT-PCR analysis indicated that four of the isolated genes were expressed in a cell cycle-dependent manner. Our results demonstrate that fission yeast system can be efficiently used to identify the genes that may function, either positively or negatively, in the regulation of cytokinesis. More importantly, the candidate genes we have isolated in this work can provide useful information for unraveling the regulators controlling cell separation at the late stage of BY-2 cell division.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据