期刊
ANALYTICAL BIOCHEMISTRY
卷 462, 期 -, 页码 19-25出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2014.01.010
关键词
Fluorescent sensor; Lead ion detection; G-quadruplex
资金
- National High Technology Research and Development Program (863 Program) of China [2012AA101405]
- Special Fund for Agro-Scientific Research in the Public Interest of China [200903056]
- National Natural Science Foundation of China [31071860]
- Shanghai Natural Science Foundation [13ZR1421700]
A label-free fluorescent DNA sensor for the detection of lead ions (Pb2+) based on lead(II)-stabilized G-quadruplex formation is proposed in this article. A guanine (G)-rich oligonucleotide, T30695, was used as a recognition probe, and a DNA intercalator, SYBR Green I (SG), was used as a signal reporter. In the absence of Pb2+, the SG intercalated with the single-stranded random-coil T30695 and emitted strong fluorescence. While in the presence of Pb2+, the random-coil 130695 would fold into a G-quadruplex structure and the SG could barely show weak fluorescence, and the fluorescence intensity was inversely proportional to the involving amount of Pb2+. Based on this, a selective lead ion sensor with a limit of detection of 3.79 ppb (parts per billion) and a detection range from 0 to 600 ppb was constructed. Because detection for real samples was also demonstrated to be reliable, this simple, low-cost, sensitive, and selective sensor holds good potential for Pb2+ detection in real environmental samples. (C) 2014 Elsevier Inc. All rights reserved.
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