期刊
PROTEOMICS
卷 7, 期 14, 页码 2340-2349出版社
WILEY
DOI: 10.1002/pmic.200600184
关键词
2-DE; AnchorChip target; liver proteome; MALDI-TOF-MS; sample preparation
An improved method for sample preparation for MALDI-MS and MS/MS using AnchorChip (TM) targets is presented. The method, termed the SMW method (sample, matrix wash), results in better sensitivity for peptide mass fingerprinting as well as for sequencing by MS/MS than previously published methods. The method allows up-concentration and desalting directly on the mass spectrometric target and should be amenable for automation. A draw back caused by extensive oxidation of methionine and tryptophan in the SMW method can be alleviated by the addition of n-octyl glucopyranoside and DTT to the sample solution. The method was validated for protein identification from a 2-DE based liver proteome study. The SMW method resulted in identification of many more proteins and in most cases with a better score than the previously published methods.
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