期刊
BIOPHYSICAL JOURNAL
卷 93, 期 1, 页码 L7-L9出版社
BIOPHYSICAL SOCIETY
DOI: 10.1529/biophysj.107.107573
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资金
- NIDCD NIH HHS [R01-DC-2775, R01 DC002775] Funding Source: Medline
- NIDDK NIH HHS [R90 DK071504] Funding Source: Medline
The voltage-dependent movement, or electromotility, of cochlear outer hair cells contributes to cochlear ampli. cation in mammalian hearing. Outer hair-cell electromotility involves a membrane-based motor in which the membrane protein prestin plays a central role. We have investigated the contribution of prestin to the mechanics and electromechanical force (EMF) generation of the membrane using membrane tethers formed from human embryonic kidney (HEK) cells. Several measures of membrane tether mechanics are greater in tethers pulled from HEK cells transfected with prestin when compared to control untransfected HEK cells. A single point mutation of alanine to tryptophan (A100W) in prestin eliminates prestin-associated charge movement and diminishes EMF but does not alter passive membrane mechanics. These results suggest that prestin-associated charge transfer is necessary for maximal EMF generation by the membrane.
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