期刊
ANALYTICAL BIOCHEMISTRY
卷 444, 期 -, 页码 8-15出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2013.09.019
关键词
Multiwalled carbon nanotube; Nafion; Cysteamine; Tyrosinase; Dopamine
资金
- Ege University Research Fund [12 FEN 027]
In this work, a multiwalled carbon nanotube (MWCNT)-Nafion-cysteamine (CA) modified tyrosinase biosensor brings a new and original perspective to biosensor technology intended for the development of dopamine determination. Dopamine measurements were done at 0.2 V with the amperometric method by the developed biosensor system. In addition, in this study dopamine determination was carried out by using the differential pulse voltammetry method between potentials of 0.4 and -0.15 V. In the optimization studies of the biosensor, some parameters such as optimal pH, optimal temperature, optimal enzyme amount, and effect of MWCNT concentration were investigated. Afterward, in the characterization studies, some parameters such as linearity and reproducibility were determined. In the reproducibility experiment, an average value of 1.026 mu M, a standard deviation of +/- 0.03975, and a coefficient of variation of 3.8% were determined for a 1-mu M dopamine concentration (n = 15). Determination of dopamine was carried out in drug samples by the developed biosensor. (C) 2013 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据