期刊
ANALYTICAL BIOCHEMISTRY
卷 445, 期 -, 页码 87-89出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2013.10.001
关键词
Lipid droplet size; Light microscopy; Quantitation; Digital image analysis; Open source software
资金
- German Federal Ministry of Education and Research (BMBF) [0315088]
A simple approach was developed for the quantification of lipid droplet size and frequency distribution in images acquired by standard light microscopy. Oil Red O-stained cell images were thresholded for the lipid droplet signal using the freely available imaging software ImageJ. Watershed algorithms allowed analyzing the area of each individual lipid droplet. The method was validated by the decrease in lipid droplet size of 3T3-L1 adipocytes on lowered glucose availability associated with reduced glycerol-3-phosphate dehydrogenase activity and reduced transcription of lipid droplet size markers. This approach can be easily applied using standard laboratory equipment without requiring expensive and complex instrumentation. (C) 2013 Elsevier Inc. All rights reserved.
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