期刊
ANALYTICAL BIOCHEMISTRY
卷 466, 期 -, 页码 16-18出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2014.08.002
关键词
Loop-mediated isothermal amplification; Conductivity; Monitor biochemical reaction; Gene analysis method
资金
- National Natural Science Foundation of China [21005086]
Loop-mediated isothermal amplification (LAMP) yields a large amount of DNA, as well as magnesium pyrophosphate precipitate, causing a decrease in ionic strength that can be measured with a conductivity meter. There is a clear relationship between the conductivity of the LAMP mixture solution and the duration of biochemical reaction. Moreover, there is also a clear relationship between the change in conductivity and the amount of initial template DNA over the range of 0.08 to 3.2 ng. These results demonstrate the feasibility not only for detecting the LAMP product qualitatively but also for real-time monitoring the biochemical reaction progression quantitatively using conductivity measurements. (C) 2014 Elsevier Inc. All rights reserved.
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