Stain-Free total protein staining is a superior loading control to β-actin for Western blots

Semi-quantification of proteins using Western blots typically involves normalization against housekeeping genes such as beta-actin. More recently, Ponceau S and Coomassie blue staining have both been shown to be suitable alternatives to housekeeping genes as loading controls. Stain-Free total protein staining offers the advantage of no staining or destaining steps. Evaluation of the use of Stain-Free staining as an alternative to beta-actin or the protein stain Ponceau S showed that Stain-Free staining was superior to beta-actin and as good as or better than Ponceau S staining as a loading control for Western blots. (C) 2013 Elsevier Inc. All rights reserved.