期刊
ANALYTICAL BIOCHEMISTRY
卷 436, 期 1, 页码 45-52出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2013.01.011
关键词
Metallothioneins; Thymine-rich oligonucleotide; Mercury; Colorimetric sensing; Gold nanoparticles
资金
- National Natural Science Foundation of China [21177052]
- Science and Technology Program of Hunan Province in China [2010SK3039]
- Key Discipline (Public Health and Preventive Medicine) in Hunan Province
A simple and sensitive method for label-free, colorimetric detection of metallothioneins (MTs) has been developed by using a thymine (T)-rich oligonucleotide (TRO)-Hg-AuNP system. In this colorimetric strategy, the thiol groups of MTs could interact with mercury from the T-Hg2+-T complex to release TRO, resulting in a color change of the system. The response signals linearly correlated with the concentration of MTs over the range of 2.56 x 10(-8) to 3.08 x 10(-7) mol L-1, and the limit of detection was 7.67 x 10(-9) mol L-1. The relative standard deviation and the recovery were 2.3-4.8% (n = 11) and 94.2-103.9%, respectively. The proposed method avoids the label and derivatization steps in common methods, allows direct analysis of the samples by the naked eye without costly instruments, and is reliable, inexpensive, and sensitive. (C) 2013 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据