期刊
ANALYTICAL BIOCHEMISTRY
卷 440, 期 2, 页码 117-119出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2013.05.019
关键词
Membrane protein; Fluorescent substrate; Hydrophobic interaction
资金
- Agence Nationale de la Recherche [ANR-2010-BLAN-1535]
- Region Ile-de-France [DIM-Malinf 110058]
We describe an unexpected feature of the dye Hoechst 33342 that may lead to misinterpretation of fluorescence assay results. When dissolved in aqueous solvent, Hoechst 33342 massively adsorbs on the polytetrafluoroethylene (PTFE)-coated stirrer and on the quartz cuvette. The interaction between the dye and the PTFE is stronger than that between the dye and the quartz. We show that Hoechst 33342 adsorption on quartz is due to its amphiphilic properties, as it depends on the buffer ionic strength and on pH. We suggest a procedure to detect and remove any residual Hoechst 33342. (C) 2013 Elsevier Inc. All rights reserved.
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