期刊
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
卷 301, 期 1-3, 页码 412-418出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.colsurfa.2007.01.012
关键词
houttuyfonate homologues; antibacterial activity; bacteria; fluidity of lipid; conformation of protein
The antibacterial activity test indicated that gram-positive (G(+)) bacteria, Staphylococcus aureus and Bacillus subtilis were more sensitive to houttuyfonate homologues (HOU-C-n) than gram-negative (G(-)) bacteria, Escherichia coli and Pseudomonas aeruginosa, and the sensitivity increased with the elongation of hydrocarbon chain of HOU-C-n. Accordingly, the effects of HOU-C-n on the membrane permeability, the lipid fluidity and the protein conformation of G(+)- and G-bacteria were investigated to elucidate the antibacterial mechanism of HOU-C-n. The results showed that no significant change in membrane permeability and fluidity of S. aureus and E. coli was observed, suggesting that the inhibition of HOU-C-n to bacteria was not caused by the interaction of HOU-C-n with membrane lipids of the bacteria. The fluorescence experiments indicated that HOU-C-n directly bound to membrane proteins of G(+)-bacteria by hydrophobic interaction, and exerted a stronger antibacterial activity. But HOU-C-n firstly interacted with proteins at the cell wall of G-bacteria, and correspondingly its effective concentration binding to the proteins of plasma membrane was greatly decreased. Therefore it exerted a weaker inhibition to G(-)-bacteria. (C) 2007 Elsevier B.V. All rights reserved.
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