期刊
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
卷 35, 期 5, 页码 1266-1272出版社
SPANDIDOS PUBL LTD
DOI: 10.3892/ijmm.2015.2145
关键词
miR-518a-3p; colorectal cancer; nuclear factor-kappa B-inducing kinase; nuclear factor-kappa B activation
资金
- Science and Technology Program
- Division of Clinical Epidemiology, First Hospital of Jilin University Dr Jiang's laboratory
The aim of the present study was to investigate the biological role and underlying mechanisms of action of miR-518a-3p in the progression and invasion of colorectal cancer (CRC). Reverse transcription-quantitative PCR (RT-qPCR) was used to examine the mRNA expression levels of miR-518a-3p in 5 CRC cell lines (SW480, SW620, HCT116, HT29 and LoVo) in a normal colonic cell line, NCM460, as well as in tumor tissues with or without metastases. The biological effects of miR-518a-3p were assessed in the CRC cell lines by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and flow cytometric analysis, and RT-qPCR and western blot analyses were employed to evaluate the expression of miR-518a-3p targets. The regulation of NF-kappa B-inducing kinase (NIK) by miR-518a-3p was confirmed using luciferase activity assays. Our results revealed that miR-518a-3p was significantly downregulated in the CRC cell lines compared with the normal colonic cell line (P<0.05), as well as in the CRC tissues with distant metastases compared with the tissues without metastases. The downregulation of miR-518a-3p was associated with tumor size, distant metastasis and TNM stage in the patients with CRC. Moreover, the ectopic expression of miR-518a-3p and the inhibition of NIK by RNA interference markedly reduced cell proliferation and enhanced the apoptosis of CRC cells. Further experiments revealed that NIK, a regulator of NF-kappa B, was a downstream target of miR-518a-3p. The presents findings indicate that miR-518a-3p plays an important role in the progression of CRC by targeting NIK.
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