期刊
ANALYTICAL BIOCHEMISTRY
卷 434, 期 1, 页码 199-201出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2012.11.009
关键词
MiRNA; Nanoengineered gold shells; LDI-TOF MS; Self-assembled monolayers
资金
- Basic Science Research Program [2009-0064280]
- Priority Research Centers Program through the National Research Foundation of Korea [2012-0006686]
- Ministry of Education, Science, and Technology
We report on a simple, fast, and highly sensitive method that allows femtomolar detection of microRNAs (miRNAs) without the need of polymerase chain reaction using a nanoengineered micro gold shell (mu AuS) and laser desorption/ionization time-of-flight (LDI-TOF) mass spectrometry (MS). Target miRNAs are selectively captured by a chemically decorated gold chip and a mu AuS, which carries with it a large number of small molecules, termed amplification tags (Am-tags). Subsequent LDI-TOF MS analysis allows a highly amplified mass signal of Am-tags for the presence of target miRNAs in a sample, resulting in ultrasensitive detection. (C) 2012 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据