期刊
ANALYTICAL BIOCHEMISTRY
卷 421, 期 2, 页码 673-679出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2011.11.008
关键词
NaYF4:Yd/Er/Gd nanoparticles; Up-conversion luminescence; Stober method; Luminescence resonance energy transfer; Phycoerythrin
资金
- National Natural Science Foundation of China [20875011]
- Northeastern University on PhD students
- U.S. National Science Foundation [DMR-0847758, CBET-0854414, CBET-0854465]
- National Institutes of Health [R21EB009909-01A1, R03AR056848-01, R01HL092526-01A2]
- Oklahoma Center for the Advancement of Science and Technology [HR11-006]
- Directorate For Engineering
- Div Of Chem, Bioeng, Env, & Transp Sys [0854465] Funding Source: National Science Foundation
- Directorate For Engineering
- Div Of Chem, Bioeng, Env, & Transp Sys [0854414] Funding Source: National Science Foundation
- Division Of Materials Research
- Direct For Mathematical & Physical Scien [847758] Funding Source: National Science Foundation
High-quality NaYF4:Yb/Er/Gd up-conversion nanoparticles (UCNPs) were first synthesized by a solvothermal method using rare earth stearate, sodium fluoride, ethanol, water, and oleic acid as precursors. Doped Gd3+ ions can promote the transition of NaYF4 from cubic to hexagonal phase, shorten the reaction time, and reduce the reaction temperature without reducing the luminescence intensity of NaYF4:Yb/Er UCNPs. X-ray diffraction, infrared spectroscopy, transmission electron microscopy, and luminescence spectroscopy were applied to characterize the UCNPs. The nanoparticles exhibited small size and excellent green up-conversion photoluminescence, making them suitable for biological applications. After the surfaces of NaYF4:Yb/Er/Gd UCNPs were modified with amino groups through the Stober method, they could be brought close enough to the analytically important protein called R-phycoerythrin (R-PE) bearing multiple carboxyl groups so that energy transfer could occur. A luminescence resonance energy transfer (LRET) system was developed using NaYF4:Yb/Er/Gd UCNPs as an energy donor and R-PE as an energy acceptor. As a result, a detection limit of R-PE of 0.5 mu g/ml was achieved by the LRET system with a relative standard deviation of 2.0%. Although this approach was first used successfully to detect R-PE, it can also be extended to the detection of other biological molecules. (C) 2011 Elsevier Inc. All rights reserved.
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