期刊
ANALYTICAL BIOCHEMISTRY
卷 422, 期 2, 页码 89-95出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2011.12.038
关键词
Quantitative; Real time; Polymerase chain reaction; Multiplex
资金
- Biochip Innovations via Uniquest
- office of the University of Queensland
We report the development of a new real-time polymerase chain reaction (PCR) detection system that uses oligonucleotide tagged PCR primers, a fluorophore-labeled universal detection oligonucleotides, and a complementary quenching oligonucleotide. The fluorescence signal decreases as PCR product accumulates due to the increase in detection/quencher hybrid formation as the tagged primer is consumed. We use plasmids containing the influenza A matrix gene and the porA and ctrA genes of Neisseria meningitidis as targets for developing the system. Cycle threshold (C-t) values were generated, and the sensitivity of the new system (dubbed PrimRglo) compared favorably with the commonly used SYBR green and Taqman detection systems and, unlike the latter system, does not require the design of a new dual-labeled detection oligonucleotide for each new target sequence. (C) 2012 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据