期刊
CELL
卷 130, 期 1, 页码 141-152出版社
CELL PRESS
DOI: 10.1016/j.cell.2007.05.026
关键词
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资金
- NIGMS NIH HHS [P50 GM071508-01, R01 GM077599-02, P50 GM071508, R01 GM077599-01, R01 GM077599] Funding Source: Medline
Patterning in multicellular organisms results from spatial gradients in morphogen concentration, but the dynamics of these gradients remain largely unexplored. We characterize, through in vivo optical imaging, the development and stability of the Bicoid morphogen gradient in Drosophila embryos that express a Bicoid- eGFP fusion protein. The gradient is established rapidly ( similar to 1 hr after fertilization), with nuclear Bicoid concentration rising and falling during mitosis. Interphase levels result from a rapid equilibrium between Bicoid uptake and removal. Initial interphase concentration in nuclei in successive cycles is constant ( +/- 10%), demonstrating a form of gradient stability, but it subsequently decays by approximately 30%. Both direct photobleaching measurements and indirect estimates of Bicoid- eGFP diffusion constants ( D <= 1 mu m(2)/s) provide a consistent picture of Bicoid transport on short ( similar to min) time scales but challenge traditional models of long- range gradient formation. A new model is presented emphasizing the possible role of nuclear dynamics in shaping and scaling the gradient.
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