期刊
ANALYTICAL BIOCHEMISTRY
卷 410, 期 2, 页码 310-312出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2010.11.015
关键词
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资金
- ThermoFisher Scientific, Inc.
The BCA assay is a calorimetric method for estimating protein concentration. In 96-well plates, the relationship between protein content and absorbance is nearly linear over a wide range; however, performance is reduced in lower volume. To overcome this limitation, we performed the BCA assays in opaque, white 384-well plates. These plates emit fluorescence between 450-600 nm when excited at 430 nm; thus, their fluorescence is quenched by the BCA chromophore (lambda(max) 562 nm). This arrangement allowed accurate determination of protein content using only 2 mu L of sample. Moreover, soluble flourescein could replace the white plates, creating a homogenous format. (C) 2010 Elsevier Inc. All rights reserved.
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