期刊
ANALYTICAL BIOCHEMISTRY
卷 419, 期 2, 页码 81-87出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2011.08.040
关键词
Isotopic exchange; O-18; Glycation; Amadori; Mass spectrometry
资金
- Ministry of Science and Higher Education of Poland [N N204 180340]
A nonenzymatic reaction of reducing sugars with the free amino group located at the N terminus of the polypeptide chain or in the lysine side chain results in glycation of proteins. The fragments of glycated proteins obtained by enzymatic hydrolysis could be considered as the biomarkers of both the aging process and diabetes mellitus. Here we propose a new method for the identification of peptide-derived Amadori products in the enzymatic digest of glycated proteins. The products of enzymatic hydrolysis of the model protein ubiquitin were incubated with (H2O)-O-18 under microwave activation. We observed that at these conditions the Amadori compounds selectively exchange one oxygen atom in the hexose moiety. The characteristic isotopic pattern of Amadori products treated with (H2O)-O-18 allows fast and convenient identification of this group of compounds, whereas nonglycated peptides are not susceptible to isotopic exchange. (C) 2011 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据