期刊
ANALYTICAL BIOCHEMISTRY
卷 419, 期 2, 页码 71-75出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2011.08.008
关键词
DNA; Poly(sulfosalicylic acid); Electrochemical sensor; Adenine; Guanine; Thymine
资金
- National Natural Science Foundation of China [21075029]
- Program for Excellent Youth Scholars of Innovative Research Team by the Hubei Provincial Department of Education [T201101]
- Foundation of the Hubei Key Laboratory of Pollutant Analysis and Reuse Technology (Hubei Normal University) [KY2010M01]
Poly(sulfosalicylic acid) and single-stranded DNA composite (PSSA-ssDNA)-modified glassy carbon electrode (GCE) was prepared by electropolymerization and then successfully used to simultaneously determine adenine (A), guanine (G), and thymine (T). The characterization of electrochemically synthesized PSSA-ssDNA film was investigated by scanning electron microscopy (SEM) and electrochemical impedance spectroscopy (EIS). The modified electrode exhibited enhanced electrocatalytic behavior and good stability for the simultaneous determination of A, G, and Tin 0.1 M phosphate buffer solution (PBS, pH 7.0). Well-separated voltammetric peaks were obtained among A, G, and T presented in the analyte mixture. Under the optimal conditions, the peak currents for A, G, and T increased linearly with the increase of analyte mixture concentration in the ranges of 6.5 x 10(-8) to 1.1 x 10(-6), 6.5 x 10(-8) to 1.1 x 10(-6), and 4.1 x 10(-6) to 2.7 x 10(-5) M, respectively. The detection limits (signal/noise = 3) for A, G, and T were 2.2 x 10(-8), 2.2 x 10(-8), and 1.4 x 10(-6) M, respectively. (C) 2011 Elsevier Inc. All rights reserved.
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