期刊
ANALYTICAL BIOCHEMISTRY
卷 408, 期 1, 页码 157-159出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2010.09.008
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资金
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM076127] Funding Source: NIH RePORTER
- NIGMS NIH HHS [R01 GM076127-01A1, R01 GM076127] Funding Source: Medline
Inductively coupled plasma-mass spectrometry (ICP-MS)-based assays lend themselves to multiplexing due to the high resolution between mass channels, the sensitivity, and the reliability of the technique. Here the potential of ICP-MS-based protease assays is demonstrated with a quadruplex assay of cysteine proteases and metalloproteases. Four orthogonal peptide substrates were synthesized for the proteases calpain-1, caspase-3, matrix metalloprotease-9 (MMP-9), and a disintegrin and metalloprotease-10 (ADAM10). Each substrate carries a biotin tag at the C terminus and a diethylenetriaminepentaacetic acid (DTPA)-based lanthanide complex at the N terminus. The results demonstrate that this is a simple and reproducible analysis technique with excellent correlation between the single and multiplex assay formats. (C) 2010 Elsevier Inc. All rights reserved.
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