期刊
ANALYTICAL BIOCHEMISTRY
卷 406, 期 2, 页码 105-112出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2010.07.015
关键词
Plasma; Serum; Metabolomics; Gas chromatography/time-of-flight mass spectrometry; Principal components analysis
资金
- National Key New Drug Creation Special Programs [2009ZX09304-001, 2009ZX09502-004]
- National Natural Science Foundation of the People's Republic of China [30630076]
- Jiangsu Province Social Development Foundation [BE2008673]
- National 11th 5-Year Technology Supporting Program of the People's Republic of China [2006BA108B04]
In metabolomic research, blood plasma and serum have been considered to possess similar compositions and properties. Their perceived equivalence has resulted in researchers choosing arbitrarily between serum and plasma for analysis. Here, routine serum and plasma were prepared and their low-molecular-weight compounds were determined using gas chromatography/time-of-flight mass spectrometry. Principal components analysis was applied to process the acquired data, and marked differences in metabolite profiles were observed between serum and plasma. Of the 72 identified compounds, 36 (50%) discriminate serum from plasma, with 29 and 7 metabolites showing a significantly higher abundance (t test, P < 0.05) in serum and plasma, respectively. Incubation of blood had distinct effects on the analyte peak areas, with the effects being more pronounced for plasma than for serum and more pronounced for a shorter incubation than for a longer incubation. These results highlight the importance in choosing serum or plasma as the analytical sample and in stipulating the incubation time. Because incubation affected the analyte peak areas less in serum than in plasma, we recommend serum as the sample of choice in metabolomic studies. (C) 2010 Elsevier Inc. All rights reserved.
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