期刊
ANALYTICAL BIOCHEMISTRY
卷 398, 期 1, 页码 24-33出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2009.10.048
关键词
Cellular oxygen; Intracellular oxygen-sensitive probe; Cell-penetrating peptides; Pt-porphyrins; Mitochondrial function; Hypoxia
资金
- Science Foundation of Ireland [07/IN.1/B1804]
- Science Foundation Ireland (SFI) [07/IN.1/B1804] Funding Source: Science Foundation Ireland (SFI)
Probing of molecular oxygen in mammalian cells is important for the analysis of mitochondrial function, metabolic responses, and energetic status of the cells. We describe a new panel of intracellular O-2-sensitive probes based on phosphorescent porphyrin dyes conjugated to cell-penetrating peptides. The probes comprising the uncharged derivatives of Pt(II)-coproporphyrin I covalently linked to positively charged TAT-derived peptides are shown to effectively load live mammalian cells without any transfection reagents. The probes work well with all cell types tested, show similar subcellular localization, and produce characteristic responses to cell stimulation with mitochondrial uncouplers and inhibitors. They provide a simple and versatile tool for O-2 monitoring in live cells and in tissue, and an alternative to the existing O-2 probes which require facilitated transport into the cell. (C) 2009 Elsevier Inc. All rights reserved.
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