Analysis of the effectiveness of reused primary and secondary antibodies in Western blotting analysis

Western blot analysis is a common technique for detecting a tat-get protein on a nitrocellulose membrane. The target protein is detected using specific antibodies. The positive signal can be visualized either by chemiluminescence or by a colorimetric reaction. Some specific antibodies can be produced in-house in high amounts and can be used without Considering costs. However many antibodies (especially antibodies for detection tag fused proteins) must be purchased from a company. Unfortunately most of them are very expensive. Here we report the effectiveness of different antibodies after reusing them several times and also compare the influence of different storage conditions. (C) 2009 Elsevier Inc. All rights reserved.