期刊
FEBS LETTERS
卷 581, 期 18, 页码 3422-3428出版社
WILEY
DOI: 10.1016/j.febslet.2007.06.049
关键词
CASP-9; histone H1.2; APAF-1; UV irradiation
Cytochrome c (CYT c) is a protein that employs the caspase recruitment domain (CARD)-containing proteins APAF-I and CASP-9 to activate effectors CASP-3 and -7. By using affinity labeling techniques and mass spectrometry analysis, we show that histone H1.2 is a regulator of caspases upon UV irradiation. We demonstrated that histone H1.2 forms a protein complex with APAF-I, CASP-9 and CYT c upon UV irradiation. In cell-free systems, we show that histone H1.2 triggers activation of CASP-3 and -7 via APAF-I and CASP-9. We therefore conclude that upon DNA damage histone H1.2 acts as a positive regulator of apoptosome formation. Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.
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