期刊
ANALYTICAL BIOCHEMISTRY
卷 403, 期 1-2, 页码 102-107出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2010.04.006
关键词
Cyclic imines; Spirolide; Phycotoxin; Fluorescence polarization; Detection method; Nicotinic acetylcholine receptors; Shellfish; alpha-Bungarotoxin
资金
- Ministerio de Ciencia y Tecnologia, Spain [AGL2006-08439/ALI, AGL2007-60946/ALI]
- Xunta de Galicia, Spain [GRC 30/2006, PGIDIT 07MMA006261PR, PGIDT07C-SA012261PR, PGDIT 07MMA006261PR, 2008/CP389]
- EU [IP FOOD-CT-2004-06988, CRP 030270-2, 211326-CP]
- Agence Nationale de la Recherche (France) [PCV07-194417-NEUROSPIROIMINE]
- [STC-CP2008-1-555612]
Fluorescence polarization (FP) is a powerful tool for studying molecular interactions by monitoring changes in the apparent size of fluorescent molecules. In this paper, a previously described fluorescence polarization assay was used to detect 13,19-didesmethyl C spirolide. The assay is based on the competition of cyclic imine marine biotoxins with alpha-bungarotoxin for binding to nicotinic acetylcholine receptor-enriched membranes of Torpedo marmorata. The 13,19-didesmethyl C spirolide was detected in buffer and mussel matrix. The sensitivity of the assay for the 13,19-didesmethyl C spirolide and the 13-desmethyl C spirolide was similar. After an acetone/chloroform extraction of spiked mussel meat, the average recovery rate of 13,19-didesmethyl C spirolide was 77.7 +/- 1.9%. The quantification range for this toxin in mussel was 40-200 mu g/kg of shellfish meat. This assay can be used to detect the spirolides 13,19-didesmethyl C spirolide and 13-desmethyl C spirolide, in shellfish as a screening assay. (C) 2010 Elsevier Inc. All rights reserved.
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