期刊
ANALYTICAL BIOCHEMISTRY
卷 405, 期 1, 页码 138-140出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2010.05.032
关键词
qRT-PCR; Reference gene; Brassica napus; Embryo; geNORM
资金
- Alberta Agricultural Research Institute
- Alberta Canola Producers Commission
- Natural Sciences and Engineering Research Council of Canada
- Canada Research Chairs Program
- Genome Canada
- Genome Alberta
Eight reference genes of Brassica napus were evaluated using quantitative real-time polymerase chain reaction (qRT-PCR) data, focusing on vegetative tissues and developing embryos. Analyses of expression stability indicated that UP1, UBC9, UBC21, and TIP41 were the top four choices as stably expressed reference genes for vegetative tissues, whereas ACT7, UBC21, TIP41, and PP2A were the top four choices for maturing embryos. In addition, radiolabeling of overall messenger RNA (mRNA) of maturing embryos indicated that the expression patterns of the top four ranked reference genes reflected the overall mRNA content changes in maturing embryos. Crown Copyright (C) 2010 Published by Elsevier Inc. All rights reserved.
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