期刊
ANALYTICAL BIOCHEMISTRY
卷 388, 期 2, 页码 345-347出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2009.02.003
关键词
DNA degradation; Minute amounts; DNA size determination; DNA quantification; GMO
资金
- La Region Aquitaine, le Pole Aquitaine Sante
- Oseo ANVAR
There is currently no method allowing routine characterization of minute amounts of degraded DNA samples Such is those encountered in forensic science, archived tissues, ancient DNA, extracellular or stool DNA, and processed food. Here we describe and directly validate such a method based, on the one hand, oil a generalized DNA random fragmentation model and, on the other, oil two quantitative polymerase chain reaction (PCR) experiments using two different target sizes. The model also makes it possible to determine the minimum sample amount, the minimum mass average fragment size, and the maximum degradation time necessary to obtain a positive PCR. (c) 2009 Elsevier Inc. All rights reserved.
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