期刊
ANALYTICAL BIOCHEMISTRY
卷 395, 期 1, 页码 108-110出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2009.07.045
关键词
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资金
- FCT [SFRH/BD/16296/2004/XS49]
- Portuguese Foundation for Science and Technology
Some experimental methodologies require the quantification of protein in the presence of polymers like poly(ethylene glycol) (PEG) and dextran (DEX). In the aqueous two-phase system (ATPS) extraction of biomolecules, the interference of these phase-forming polymers on the Bradford quantification assay is commonly recognized. However, how these polymers interfere has not been reported hitherto. In this study we show that while dextran concentrations of 20% (w/w) can be used without error, loss of accuracy occurs for solutions with PEG concentrations > 10% (w/w). Above this value a substantial decrease on the assay sensitivity is observed. (C) 2009 Elsevier Inc. All rights reserved.
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