期刊
ANALYTICAL BIOCHEMISTRY
卷 384, 期 1, 页码 34-41出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2008.09.019
关键词
Fluorescence; Zinc; Copper; Nickel; Manganese; Iron; Cobalt; Cadmium
资金
- NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE [R01NS047508] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE ON AGING [R01AG020899] Funding Source: NIH RePORTER
- NIA NIH HHS [R01 AG020899, AG20899] Funding Source: Medline
- NIEHS NIH HHS [P30 ES0560] Funding Source: Medline
- NINDS NIH HHS [R01 NS047508-01A1, R01 NS047508, NS47508] Funding Source: Medline
Zinc-sensitive fluorescent probes have become increasingly important in the investigation of the cellular roles of zinc. There is, however, little information on how the other transition metals in cells may influence the measurement of zinc. We have characterized in vitro the interaction of the nominal zinc indicators FluoZin-3 and Newport Green with all the cationic transition metals found within cells, Cr, Mn, Fe, Co, and Cu, as well as Ni and Cd, by measuring their dissociation constants. In addition, we have shown how FluoZin-3 can be used to quantify the concentration of copper in a cell-free assay and report that the fluorescence of Newport Green is boosted by both Cu(I) and Fe(II). Furthermore, we have introduced diagnostics for detecting the interference of metals other than zinc with its measurement within cells. (C) 2008 Elsevier Inc. All rights reserved.
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