期刊
ANALYTICAL BIOCHEMISTRY
卷 395, 期 1, 页码 116-118出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2009.07.049
关键词
-
资金
- Hope Center for Neurological Disorders at Washington University [NIA K23 AG030946, NINDS RO1-NS065667]
- Alzheimer's Disease Research [A2008-345]
- American Health Assistance Foundation
- NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE [R01NS065667] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE ON AGING [K23AG030946] Funding Source: NIH RePORTER
Using apolipoprotein E (ApoE) as a model protein, we developed a protein isoform analysis method utilizing stable isotope labeling tandem mass spectrometry (SILT MS). ApoE isoforms are quantitated using the intensities of the b and y ions of the C-13-labeled tryptic isoform-specific peptides versus unlabeled tryptic isoform-specific peptides. The ApoE protein isoform analysis using SILT allows for the simultaneous detection and relative quantitation of different ApoE isoforms from the same sample. This method provides a less biased assessment of ApoE isoforms compared to antibody-dependent methods, and may lead to a better understanding of the biological differences between isoforms. (C) 2009 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据