期刊
ANALYTICAL BIOCHEMISTRY
卷 387, 期 2, 页码 178-183出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2009.01.014
关键词
Ag(III) complex; Luminol; Chemiluminescence; Cortisol; Determination
资金
- Key Technologies Research and Development Program of Hebei Province [072761894]
A new chemiluminescence (CL) system based on the reaction of Ag(III) complex with luminol is, for the first time, reported in this work, Incorporated with a flow injection analyses (FIA), the new CL system has been applied for the determination of free cortisol in human sera. The system is based on the CL reaction of luminol with Ag(III) in alkaline solutions, while cortisol can dramatically enhance CL intensities. Under Optimum conditions, CL intensities are proportional to concentration of cortisol in the range of 0.05-7.5 nM. The limit of detection is 2.0 x 10(-11) M (3 sigma), with a relative standard deviation (n = 11) of 1.9% for 3.5 x 10(-9) M cortisol. Eight human blood serum samples were all handled by solid-phase extraction (SPE) clean-up and enrichment before detection. This detection system is highly sensitive and convenient and may find wide applications. Based on the chemiluminescent spectra, a possible reaction mechanism is also Suggested. (C) 2009 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据