期刊
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
卷 35, 期 3, 页码 724-730出版社
SPANDIDOS PUBL LTD
DOI: 10.3892/ijmm.2015.2066
关键词
short bowel syndrome; vitamin A; cellular retinol-binding protein II; lecithin retinol acyltransferase; apolipoprotein A; intestinal adaptation
资金
- JSPS KAKENHI [23592623, 25504001, 23590228]
- Grants-in-Aid for Scientific Research [23590228, 23592623, 25504001] Funding Source: KAKEN
Short bowel (SB) syndrome causes the malabsorption of various nutrients. Among these, vitamin A is important for a number of physiological activities. Vitamin A is absorbed by epithelial cells of the small intestine and is discharged into the lymphatic vessels as a component of chylomicrons and is delivered to the liver. In the present study, we used a rat model of SB syndrome in order to assess its effects on the expression of genes associated with the absorption, transport and metabolism of vitamin A. In the rats with SB, the intestinal mRNA expression levels of cellular retinol-binding protein II (CRBP II, gene symbol Rbp2) and apolipoprotein A-IV (gene symbol Apoa4) were higher than those in the sham-operated rats, as shown by RT-qPCR. Immunohistochemical analysis revealed that absorptive epithelial cells stained positive for both CRBP II and lecithin retinol acyltransferase, which are both required for the effective esterification of vitamin A. In the rats with SB, the retinol content in the ileum and the retinyl ester content in the jejunum were lower than those in the sham-operated rats, as shown by quantitative analysis of retinol and retinyl esters by high performance liquid chromatography. These results suggest that the elevated mRNA expression levels of Rbp2 and Apoa4 in the rats with SB contribute to the effective esterification and transport of vitamin A.
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