期刊
ACTA PHYSIOLOGIAE PLANTARUM
卷 29, 期 4, 页码 361-367出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s11738-007-0045-2
关键词
cowpea; enzyme activity; isoform; salicylic acid; Vigna unguiculata L. walp
In this paper, we describe a study concerning the determination of phenylalanine ammonia lyase ( PAL) activities leading to decline in disease progress caused by Rhizoctonia solani after application of salicylic acid ( SA). Two applications of 1.4 mM SA ( pH 6.5) followed by inoculation with Rhizoctonia solani resulted in a quantitative change in polyphenol oxidase (PPO), peroxidase ( PRX) isoforms and increase in PAL activities from 4.38 to 19.48 unit g(-1) (FM) h(-1) in Bundel-1, UPC-4200 and IFC-902 cowpea genotypes. Increase in PAL activities was further observed specifically in UPC-4200 when plants were exposed with Rhizoctonia solani spores. Total soluble proteins did not change after SA treatment, however they were significantly increased in SA sprayed-inoculated UPC-4200 genotype. Of the ten detected isoforms of polyphenol oxidase, isoforms 7 and 10, and isoform 4 of peroxidase showed increased activities by SA application. The disease symptoms measured as areas under progress curve (AUDPC) indicated less A value in SA sprayed Bundel-1 and UPC-4200 genotypes over their controls.
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