期刊
ANALYTICAL BIOCHEMISTRY
卷 385, 期 2, 页码 242-248出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2008.11.017
关键词
Biosensors; Oxygen sensing; Cell viability; Antibiotic screening; FRET
资金
- EdRox Marie Curie Training Network [MRTN-CT-2006-035649]
- FOM [fom-67596]
- VENI [700.54.411]
Fluorescently labeled hemocyanin has been previously proposed as an oxygen sensor. In this study, we explored the efficacy of this biosensor for monitoring the biological oxygen consumption of bacteria and its use in testing bacterial cell growth and viability of Escherichia coli, Pseudomonas aeruginosa, Paracoccus denitrificans, and Staphylococcus simulans. Using a microwell plate, the time courses for the complete deoxygenation of samples with different initial concentrations of cells were obtained and the doubling times were extracted. The applicability of our fluorescence-based cell growth assay as an antibacterial drug screening method was also explored. The results provide a proof-of-principle for a simple, quantitative, and sensitive method for high-throughput monitoring of prokaryotic cell growth and antibiotic susceptibility screening. (C) 2008 Elsevier Inc. All rights reserved.
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