期刊
ANALYTICAL BIOCHEMISTRY
卷 384, 期 2, 页码 238-244出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2008.10.015
关键词
Creatinine; Zone fluidics; Kinetic spectrophotometry; Jaffe reaction; Urine analysis
资金
- Gerber Foundation
- State of Texas Advanced Research [3644-0007-2006]
- Centers for Disease Control
Urinary creatinine analysis is required for clinical diagnosis, especially for evaluation of renal function. Creatinine adjustment is also widely used to estimate 24-h excretion from spot samples. Few convenient validated approaches are available for in-house creatinine measurement for small- to medium-scale Studies. Here we apply the Jaffe reaction to creatinine determination with zone fluidic multichannel kinetic spectrophotometry. Diluted urine sample and reagent, alkaline picric acid, were mixed by a computer-programmed dispenser and rapidly delivered to a four-channel detection cell. The absorbance change was monitored by a flow-through light-emitting diode-photodiode-based detector. Validation results against high-performance liquid chromatography-ultraviolet (HPLC-UV)/mass spectrometry (MS) are presented. Responses for 10-fold diluted samples were linear within clinically relevant ranges (0-250 mg/L after dilution). The system can analyze 70 samples per hour With a limit of detection of 0.76 mg/L. The relative standard deviation was 1.29% at 100 mg/L creatinine (n =225). Correlation with the HPLC (UV quantitation/MS confirmation) system was excellent (linear, r(2) = 0.9906). The developed system allows rapid, simple, cost-effective, and robust creatinine analysis and is suitable for the analysis of large numbers of urine samples. (C) 2008 Elsevier Inc. All rights reserved.
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