期刊
ANALYTICAL BIOCHEMISTRY
卷 389, 期 1, 页码 89-91出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2009.03.017
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资金
- NCI NIH HHS [P30 CA013330-35, P30 CA013330, R37 CA026504, R01 CA026504, CA26504, R37 CA026504-31, 5P30-CA13330] Funding Source: Medline
- NATIONAL CANCER INSTITUTE [R01CA026504, R37CA026504, P30CA013330] Funding Source: NIH RePORTER
Available protocols for stripping antibodies from immunoblots involve the use of sodium dodecyl sulfate (SDS) or low-pH buffers. SDS was shown to remove transferred proteins from membranes, and low-pH buffer was shown to inefficiently strip off antibodies. A solution containing 6 M guanidine hydrochloride, 0.2% nondenaturing detergent, and a reducing agent can rapidly strip off tightly bound antibodies from) aged polyvinylidene fluoride (PVDF) immunoblots at room temperature Without removing significant amounts of transferred protein. (C) 2009 Elsevier Inc. All rights reserved.
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